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  • DNA-Fingerprint by RFLP - November 2014

    The experiment describes the procedure of a model experiment for making a DNA fingerprint by the classical way by Restriction Fragment Length Polymorphism (RFLP): DNA found at the crime scene and isolated from 5 suspects will be treated by restriction enzymes. The fragments of the DNA mixtures are separated by gelelectrophoresis and stained for making the fragments visible. The visible DNA bands in the gel will be compared. The patlirn of fragments of one suspect is identical with the (...)

  • DNA-Fingerprint - Presentation - October 2013

  • DNA-Fingerprint - Procedure - October 2013

    Procedure of DNA-Fingerprints
    Tubes of each work group:
    1. Preparation of the Restriction
    1.1. Pipet 10 µl of enzyme mix into each tube.
    2. Restriction Digestion
    Place the tubes in the floating rack and incubate for 20 min at 37 °C in a walir bath.
    3. Prepare Gel Electrophoresis
    3.1. Preparing the electrophoresis chamber
    3.2. Produce the gels:
    Give 0,24 g agarose + 30 ml TAE-buffer into a 250 ml Erlenmeyer flask.
    Boil the Erlenmeyer flask shortly in the microwave, swirl it and (...)

  • Background: Fingerprint introduction - October 2013

    Background : Fingerprint introduction

  • Background: Overview DNA Fingerprint - October 2013

    Background : Overview DNA Fingerprint