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Published 12 February 2015

Protocol for the determination of the origin of the contamination of the patient

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Here, you have three files of procedure for the determination of the origin of the contamination of the patient in word, in pdf, and the ppt for the presentation of this experiment.

Determination of the origin of the contamination of the patient- Protocol in word
Determination of the origin of the contamination of the patient - Protocol in pdf
Determination of the origin of the contamination of the patient - Practical training in ppt
Warning ! Opening of the tubes and Agar Petri dish is realized in asepsis around Bunsen burner

1. Detection of bacterial contaminants on compresses

1.1 Open a bag of compresses.

1.2 Apply on the compresse, a Agar « contact » Petri dish. Press lightly and maintain contact approximately 10 seconds.

1.3 Use eventually a forceps to peel off the compresse and after close the box.

1.4 Write on the bottom of the box « Box 1 », and the number of your table. Incubate 24 hours at 37°C.

2. Detection of bacterial contaminants in physiological cleaning water

2.1 Open the « PW » bottle, and with a sterile pipette, sample 0,5 mL.

2.2 Put down 2 or 3 drops on the surface of the Agar Petri dish.

2.3 With a sterile rack, spread the drops on the surface of the agar.

2.4 Close the box. Write on the bottom of the box « Box 2 », and the number of your table. Incubate 24 hours at 37°C.

3. Detection of bacterial contaminants in a catheter

3.1 Take the catheter with a forceps, in approximately 1 cm of the extremity.

3.2 With a swab, rub inside the catheter next to this extremity.

3.3 With the swab, realize a spreading on the surface of the agar plate dish. Realize tight streaks.

3.4 Close the box. Write on the bottom of the box « Box 3 », and the number of your table. Incubate 24 hours at 37°C.

4. Detection of bacterial contaminants in perfusion liquid

4.1 On the pre-installed filtering system, check the presence of a bacteria filter.

4.2 Pour the content of the perfusion liquid (PL) on the filter. Be carefull : not to put drops on the walls of the filtering system.

4.3 Run the pression on the filtering system, to triger vacuum, until filter all the liquid.

4.4 Take off the upper part of filtering system, so as to access to the filter.

4.5 With a forceps, recover the filter and deposit it immediately on a small agar Petri dish
Apply a light pression with the forceps to allow a good adhesion of the filter on the agar.

4.6 Close the box. Write on the bottom of the box « Box 4 », and the number of your table. Incubate 24 hours at 37°C

5. Results

The second day, after incubation, observe the surface of differents agar Petri dish to look for bacterial colonies.

What is the probable origin of the contamination ?