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Published 28 November 2014

Detection of genetically modified food by PCR

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The experiment describes the investigation procedure if food is genetically modified or not. After DNA isolation out of food, specific sequences of the cauliflower mosaic virus promoter and the NOS terminator which are very often used for the regulation of the foreign gen, were amplified via PCR. The amplified DNA sequences will be separated by agarose gelelectrophoresis and made visible in the gel by staining.
If the CAMV Promoter or the NOS terminator were used in the gen modification process you can find a band in the gel at 455 bp.
Investigation results:
In all the food samples produced in Europe we can`t find a gen modification, but in food bought in American grocery stores round about 80 percent of the investigated food samples was genetically modified.
Here you will find the procedures of the experiment.

Overview of the experiment:

Overview PCR experiment
Generation of transgenic plants

Here you will find the test protocols of the experiment:
Step 1: DNA isolation and PCR:

PCR protokoll
PCR protokoll
PCR mentor manual
PCR introduction

Step 2: Detection of PCR products by electrophoresis:

Detection of PCR Products by Agarose Gel Electrophoresis
Detection of PCR Products by Agarose Gel Electrophoresis
Detection of PCR Products by Agarose Gel Electrophoresis

If you want to know more...

Applications of PCR
PCR principle
Generation of Transgenic Plants